Alpha Lifetech Inc.’s Post

Investigate intracellular bacterial pathogen mediated manipulation of host cellular functions- through live-cell imaging! https://lnkd.in/gDgawDxN ✨ This method by Madalina Mocanita, Kailey Martz, Vanessa D'Costa at University of Ottawa provides a quantitative assessment of how Salmonella manipulates host epithelial cell trafficking to lysosomes using a fluorescent marker, DQ-Red BSA. ✨This live-cell imaging assay can be adapted to study various pathogens and host cell types, offering insights into pathogenesis mechanisms and potential therapeutic targets. Click on the link 🔗 to learn more ! This work has been reviewed by Jinglin Lucy Xie and Xin Xu. #Pathogenesis #bacterial #LysosomalPathway #microbiology Do you have a protocol that can contribute to scientific clarity and reproducibility ? Visit us at https://lnkd.in/dt_igxRi and explore publishing with Bio-protocol at https://lnkd.in/gR3QGYz6! #openaccess #reproducibility #lifesciences #research

It's the last day of posters today at #HUPO2024 and there are still many worth seeing. On day 3, here are our top picks: Immunopeptidomics (P-III-1089) Thunder-DDA-PASEF enables the identification of mRNA splicing-derived immunopeptides in human tumor cell lines David Gomez-Zepeda   (P-III-1095) Dia-PASEF for in-depth immunopeptidomics analysis: challenges and new opportunities Daniel Hornburg, PhD   (P-III-1100) Fine tuning of ddaPASEF methods improves the immunopeptidomics coverage and throughput, which is further boosted by ML-assisted rescoring Jeewan Babu Rijal   Chemical Biology Insights (P-III-0874) High-throughput PROTAC compound screening workflow for targeted protein degradation on an Orbitrap Astral mass spectrometer with accurate label-free quantitation Kevin Yang   (P-III-0868) The timsTOF Ultra enables deep global ubiquitinomics of ultra-low protein input samples for validating degraderdrug targets Philipp Strohmidel For more - https://lnkd.in/gDWuZ845

For all my #flowcytometry connections. This is a pretty cool application showing the power of what cell barcoding can do with flow cytometry. Imagine not needing to worry about compromising between data quality and marker selection. Even better to get surface, intra-cellular and intra-nuclear biomarkers on the same cell without losing any data due to harsh fixatives and perms. I'll write more later about why I joined LASE Innovation Inc. to help them work on the potential of this single-cell technology... But it definitely had something to do with turning conventional flow cytometry on its head!

Giving insights of one's proper work to the whole staff: From COSBI's series: Insider talks this time Pranami Bora shared the results of her analyses of transcriptomic data from in vitro models of Idiopathic Pulmonary Fibrosis (IPF). This is what it is about: - How in vitro models mimic the fibrotic niche - How transcriptomic profiles change over time This approach contributes to a better understanding fibrosis, potentially aiding drug research. Let’s keep pushing the boundaries of fibrosis research! Pranami is a member of the #Systems #Biology Group, led by Silvia Parolo, and where there are also the researchers Nicola Casiraghi and Lorena Leonardelli. Good work to you all! #IPF #Transcriptomics #InVitroModels #DrugDiscovery #ResearchInnovation

𝗗𝗶𝗱 𝘆𝗼𝘂 𝗺𝗶𝘀𝘀 𝗼𝘂𝗿 𝗡𝗮𝗻𝗶𝗼𝗻 𝗨𝘀𝗲𝗿 𝗠𝗲𝗲𝘁𝗶𝗻𝗴? No worries. We’ve got you covered with on-demand recordings! This year, our theme was "All the Small Things". From organelles to tissues, and molecular mechanisms to nanosensing, the meeting explored research insights into the "small things" that are driving groundbreaking discoveries. Explore talks on topics such as: ▪️ High-throughput phenotyping of cardiac ion channel variants ▪️ KV3 channel positive allosteric modulators: a precision medicine approach for progressive myoclonic epilepsy ▪️ Cell-free systems for membrane protein production and functional characterization ▪️ Real-time peptide differentiation using nanopore technology ▪️ Pore-forming toxins: uncovering molecular mechanisms and nanosensing applications ▪️ Structure-based analysis of ENaC activation by proteolysis and ligands ▪️ Profiling GPCR pharmacology through impedance monitoring of cell-based assays ▪️ PI-(3,5)P2-mediated oligomerization of the endosomal Na+/H+ exchanger NHE9 Catch up on insightful talks, and stay inspired. Watch now: https://ow.ly/IgMT50UaOy8 #NanionUserMeeting #ScientificMeeting #APC #CellAnalytics #MembraneBiophysics

The One-Tip Evotip workflow is simplifying proteomics. A recent publication in Nature Communications shows that One-Tip enables high-sensitivity, single-step proteome analysis from as few as 20 cells or even single cells. With this streamlined Evotip approach, we achieve deep proteome coverage, boosting throughput and precision without complex handling. From early embryos to extracellular vesicles, One-Tip is making powerful proteomics accessible and scalable. Learn more about the one tip workflow in todays webinar: https://lnkd.in/dPC9mfEw #Evosep #Proteomics #SingleCell

Must-Read Research! Explore how a broken heart can be healed! Scientists from UBC, Borea Therapeutics, and Aspect Biosystems have uncovered region-specific mechanisms driving #heart repair after a #heartattack using 10x Genomics #Visium, advancing #therapeutic #angiogenesis strategies. Learn more: https://zurl.co/9XPCz

🌟 Excited to share our latest preprint, a collaboration with Kanishk Jain and Brian D. Strahl labs! Chromatin-associated proteins are key players in cell function and disease, yet most remain uncharacterized. Understanding how these proteins bind chromatin could transform drug development and uncover new therapeutic targets. In this study, we explored the PHD protein PHRF1 and uncovered its critical roles in splicing and the DNA damage response. Using EpiCypher's nucleosome technologies and dCypher screening assays, we defined how PHRF1 interacts with physiological chromatin substrates. Importantly, cancer-associated mutations disrupted these interactions, highlighting the pivotal role of PHD domains in chromatin function. This work showcases how cutting-edge tools like EpiCypher nucleosomes and dCypher technology are driving discovery in the chromatin field. 🎯 📄 Read the preprint: https://lnkd.in/ek6t6yuR #DNAdamage #chromatin #epigenetics #biomedicalresearch #biotechnology

🚀 New Application Note Release! 🌟 We’re excited to share our latest collaborative work with Molecular Devices, showcasing an all-optical approach for assessing the long-term drug effects on human iPSC-derived cardiomyocytes. The method combines:  💡 Genetically Encoded Calcium Indicators (GECIs) for non-toxic, extended monitoring of chronic cardiac effects 💡 Optogenetic Actuators to precisely control cardiomyocyte contractions and simulate various physiological conditions 💡 High-Content Imaging Systems for robust, high-throughput data acquisition and analysis The note highlights how combining advanced imaging with genetic engineering can enhance cardiotoxicity screening and therapeutic evaluation, paving the way for more efficient drug discovery and personalized medicine. 🧬💊 👏 Kudos to the brilliant minds at Molecular Devices and LumiSTAR for pushing the boundaries of cardiac research! Check out the full application note to explore this cutting-edge methodology. #DrugDiscovery #Cardiotoxicity #Optogenetics #StemCellResearch

Development of a #Nanopore panel for analysis of repeat expansions. The authors at the Medical Genetics Center in Munich present an updated Clinical Nanopore Cas9-Targeted Sequencing (Clin-CATS) workflow for analysing repeat expansions using R10 chemistry that incorporates the wf-human-variation Epi2Me workflow and the Straglr tool to analyse basecalling reads. They expanded their previous panel from 10 to 27 genes for ataxias. The validation is carried out on 17 Coriell reference samples and 6 clinical samples. Quote from the publication "The workflow demonstrates strong adaptability with expanded panels, improved sequencing accuracy, and bioinformatic flexibility, supporting its long-term application in research and clinical diagnostics [in Germany]" https://lnkd.in/eEQJjcHh