Enhanced antibacterial activity of carbon dots functionalized with ampicillin combined with visible light triggered photodynamic effects

Colloids Surf B Biointerfaces. 2018 Oct 1:170:347-354. doi: 10.1016/j.colsurfb.2018.06.040. Epub 2018 Jun 19.

Abstract

In the last years, carbon-based nanomaterials have attracted considerable attention in a wide range of fields, particularly in biomedicine, owing to their remarkable photo-physical and chemical properties. In this study, we demonstrate that amine-terminated carbon dots (CDs-NH2) functionalized with ampicillin (AMP) offer a new perspective for antibacterial treatment. The amine-functionalized carbon dots were used as a carrier for immobilization and delivery of ampicillin (CDs-AMP) and as a visible light-triggered antibacterial material. Additionally, AMP immobilization on the CDs-NH2 surface improves its stability in solution as compared to free AMP. The AMP conjugated CDs platform combines the antibacterial function of AMP and conserves the intrinsic theranostic properties of CDs-NH2. Therefore, the AMP immobilized onto CDs-NH2 surface together with the generation of moderate quantities of reactive oxygen species under visible light illumination are very effective to inactivate the growth of Escherichia coli.

Keywords: Ampicillin; Bactericidal activity; Carbon dots; Escherichia coli; PDT.

MeSH terms

  • Adult
  • Amines / chemical synthesis
  • Amines / chemistry
  • Amines / pharmacology*
  • Ampicillin / chemistry
  • Ampicillin / pharmacology*
  • Anti-Bacterial Agents / chemical synthesis
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Carbon / chemistry
  • Carbon / pharmacology*
  • Cell Death / drug effects
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Escherichia coli K12 / cytology
  • Escherichia coli K12 / drug effects*
  • Female
  • HeLa Cells
  • Humans
  • Light*
  • Microbial Sensitivity Tests
  • Photochemotherapy
  • Quantum Dots / chemistry*
  • Structure-Activity Relationship
  • Tumor Cells, Cultured

Substances

  • Amines
  • Anti-Bacterial Agents
  • Carbon
  • Ampicillin