Southern RNA 🧬’s Post

Along with enzymatic engineering of T7RNAP (https://lnkd.in/eJvwz5Yr), optimization of transcription reactions is another way to decrease dsRNA impurities during IVT #mRNA, #mRNAvaccines

Want to tackle dsRNA contamination in mRNA Production? We developed an optimized mRNA manufacturing process that decreases dsRNA levels in a way that is compatible with large-scale GMP-grade manufacturing and results in ultra-pure mRNA that avoids activation of the innate immune system.   Senne Dillen, Senior Scientist Drug Substance Process Development, explains how our method works in his latest article. Download it now! 👉 https://lnkd.in/eT9_H5ES #RNA #mRNAManufacturing #innovation

#Biopharma scientists, we've got a treat for you with a new application spotlight! Explore how Biozen dSEC-7 effectively separates and quantifies Cas9 IVT mRNA aggregates, following the latest USP guidelines. Optimized for high-throughput screening and precise aggregate analysis. Download it here: https://bit.ly/3BNXE2S Watch the accommodating webinar to get the full scoop: https://bit.ly/3U5emkx #Biotech #mRNA

New classes of medicines always face challenges as their use increases. For #mRNA, quality, purity and manufacturing standards are increasingly in the spotlight, with one of those concerns being capping efficiency. As Clare Whitewoods discusses in the second part of a two-part discussion on Getting #IVT Right, there are new options using RNA polymerase that can help with capping obstacles. https://ald.bio/3YjkneA

Comparing heterogeneity in mRNA-LNP formulations: Frank DeRosa and collaborators at Sanofi mRNA Center of Excellence established a reliable sucrose gradient ultracentrifugation protocol to separate subpopulations of mRNA-LNPs based on density https://lnkd.in/exXeWwK4 LNPs were segregated into two fractions based on density, with the majority of the payload and lipid content concentrated in the upper fraction in comparison to the lower fraction. Their work allowed to establish the biophysical traits of parent mRNA-LNPs and their subpopulations. An article also authored by Amita Vaidya, Ph.D., Dipen Parande, Nikita Khadse, Natalia Vargas Montoya, Vikram A., Christian Ortiz, Gordon Ellis, Neha Kaushal, Ashish Sarode and Shrirang Karve #LNP #mRNA #formulation #drugdelivery #Vesiculab

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Have you heard about the higher activity of the new RNA capping enzyme from New England Biolabs and the improved approach for LC-MS mRNA cap analysis? https://lnkd.in/eppPzaBw 💡 Learn about Faustovirus Capping Enzyme (FCE) advantages over Vaccinia Capping Enzyme (VCE) for mRNA manufacturing, and how RNase 4 simplifies mRNA cap LC-MS analysis design in the latest NEB blog post.

Transforming human iPSCs into hepatic cells requires tailored conditions at each differentiation phase, utilizing media enriched with particular growth factors and cytokines. Leveraging Sartorius Research Grade Growth Factors and Cytokines in combination with Incucyte®️ Live-cell Analysis and iQue®️3 High-Throughput Screening by Cytometry, we've established a straightforward protocol for creating mature hepatic organoids, streamlining the production of functional liver cells from iPSCs for research in drug development and toxicity testing. Learn more in our application note: https://ow.ly/3sxc50RzCfZ #ipscs #organoids #drugdiscovery #cellanalysis #livecellanalysis

Uncover the Power of UV/Vis #Spectroscopy in biopharma workflows! Join us for a free webinar on May 15th, 2024 at 3:00 PM BST to explore how UV/Vis spectroscopy is transforming biopharma workflows for enhanced quality and regulatory compliance. 👉>>> https://bit.ly/3x63tpS Key Takeaways: Optimize nucleic acid sample quality for PCR and NGS techniques. Gain insights into best practices and compliance tips from industry experts. Learn from Justin Shaffer, Product Marketing Specialist at Thermo Fisher Scientific, as he shares his experience in regulated environments. Register now to elevate your biopharma game! #biopharma #UVVis

05-2024. And here is #2: "Evaluating the Potential of Cyclodextrins in Reducing Aggregation of Antibody–Drug Conjugates with Different Payloads". Again, free download, JPharmSci! Just follow the link: https://lnkd.in/dwFzhgbi