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𝗠𝗶𝗰𝗿𝗼𝗯𝗶𝗼𝗹𝗼𝗴𝘆 𝟭𝟬𝟬 𝗶𝗻𝘁𝗲𝗿𝘃𝗶𝗲𝘄 𝗾𝘂𝗲𝘀𝘁𝗶𝗼𝗻𝘀 𝟵𝟲. 𝗘𝘅𝗽𝗹𝗮𝗶𝗻 𝗮𝗯𝗼𝘂𝘁 𝗵𝗼𝘄 𝘁𝗼 𝗰𝘂𝗹𝘁𝘂𝗿𝗲 𝘀𝘁𝗼𝗿𝗮𝗴𝗲 𝗮𝗻𝗱 𝗵𝗮𝗻𝗱𝗹𝗶𝗻𝗴? 𝗖𝘂𝗹𝘁𝘂𝗿𝗶𝗻𝗴 Bacteria are cultured based on specific growth conditions, including temperature, oxygen requirements, and medium. Cultures can be started from frozen, lyophilized, or live samples. For example, frozen vials should be thawed quickly and transferred to growth medium, while lyophilized cultures are rehydrated and inoculated into fresh media. 𝗦𝘁𝗼𝗿𝗮𝗴𝗲 Storage methods depend on the preservation form. For long-term storage, cryopreservation (using cryoprotectants like glycerol or DMSO) is recommended, often in liquid nitrogen for viability. Alternatively, freeze-dried cultures can be kept at 4°C but need moisture protection due to their hygroscopic nature. 𝗛𝗮𝗻𝗱𝗹𝗶𝗻𝗴 Cultures must be handled aseptically to avoid contamination, and specific safety protocols are followed based on biosafety levels. The guide suggests using biosafety cabinets and sterilizing equipment when handling potentially infectious cultures. These procedures ensure bacterial viability and minimize contamination risks during storage and handling. #microbiology #pharma #culture #atcc #vijaykumarh

Facing challenges with your soy peptone supply chain? Our extensive network helps to ensure uninterrupted availability of premium soy peptones, tailored to meet the rigorous demands of biotech and diagnostic sectors. Access exclusive insights on our robust inventory of soy peptones: https://lnkd.in/e37sHXyZ #KerryLifeScience #Diagnostics #SupplyChainManagement

How do LifeCheck ATP Test Kits work with produced waters? The entire test takes 5-10 minutes, the procedure involves: 1. Drawing the sample into the supplied syringe and passing it through a filter to remove any debris and trap the cells on the filter 2. A rinsing step to remove contaminants 3. Passing the Reagent X through the filter to break open the cells, releasing the ATP for analysis 4. The solution collected after passing Reagent X through the filter contains the ATP 5. Mixing the resulting liquid with Reagent Z that contains an enzyme that uses this ATP to emit light 6. Measure the amount of light (RLU’s) using the LifeCheck PhotonMaster 7. An easy-to-use calculator tool then converts the RLU reading to a measure of microbial load (Microbial Equivalents) Learn more at: https://buff.ly/3GlRUwW #osppolytechnic #oilandgasmicrobiology #microbiology101 #atptestingcertification #ospmicrocheck

Some of the key products we offer include: Kauffman Tetrathionate (MKTTn Broth Media): Ideal for the enrichment of Salmonella species. Hekton Enteric Agar: A selective medium for isolating enteric pathogens. Fraser Broth: Used for the enrichment of Listeria monocytogenes. E.C. Broth with MuG: For the detection of E. coli. Bacillus cereus Agar: A selective medium for the isolation of Bacillus cereus. Listeria Selective Supplement: For enhancing growth and differentiation of Listeria species. Sorbitol MacConkey Agar (sMac Agar): Used for isolating E. coli O157:H7. Deoxycholate Citrate Agar (DCA): For the selective isolation of enteric pathogens. Brain Heart Infusion Agar (BHIA) & Brain Heart Infusion Broth (BHIB): Enriched media for the cultivation of fastidious organisms. Bile Aesculin Agar: For identifying enterococci and group D streptococci. DNAse Agar: For the detection of DNAse activity in bacteria. Urea Agar Base: For testing urease activity. Lysine Iron Agar & Kligler Iron Agar: Used for the identification of enteric bacteria. MacConkey Agar without Salt or Crystal Violet: For non-selective isolation of Gram-negative bacteria. Columbia Agar Base: A rich medium for growing a wide range of microorganisms. Sabouraud Chloramphenicol Agar: Ideal for fungal cultures. CLED Agar: Cysteine-Lactose-Electrolyte-Deficient Agar for the isolation of urinary pathogens. These products are designed for a wide array of applications, including food safety testing, clinical diagnostics, environmental testing, and more. At Babio, we are committed to providing premium-quality products that ensure the reliability and accuracy of your laboratory results. Visit our website to explore our full range of products: www.babiolab.com WhatsApp：008613365418940 #Babio #Microbiology #LaboratoryMedia #Biotechnology #ScientificResearch #MicrobialTesting #Health #Diagnostics #LifeSciences #MedicalResearch #FoodSafety #ClinicalTesting #Bacteriology #Innovation

I recently conducted an exciting microbiological practical, isolating Escherichia coli from cow dung, a common source of this bacterium. The experiment provided valuable insights into microbial isolation and pathogen detection techniques. Below is an overview of the process: 1. Sample Collection and Preparation: Fresh cow dung was collected and suspended in sterile saline to create a homogenous sample. 2. Serial Dilution: A series of dilutions were prepared to reduce the microbial load, ensuring that individual colonies could be isolated. 3. Selective Culturing: Diluted samples were inoculated onto Eosin Methylene Blue (EMB) agar plates. This selective medium helps differentiate E. coli due to its ability to ferment lactose, resulting in characteristic colony formation. 4. Incubation: The inoculated plates were incubated at 37°C for 24-48 hours to promote bacterial growth. 5. Colony Identification: After incubation, I observed colonies exhibiting the characteristic green metallic sheen on EMB agar, a hallmark of E. coli presence. This was an important confirmation of successful isolation. 6. Biochemical Testing: To further validate the identification, additional tests like Gram staining and biochemical tests (e.g., Indole, Methyl Red) can be performed to confirm the identity of E. coli. This practical experience reinforced key laboratory techniques, including aseptic handling, bacterial culturing, and the importance of selective media in microbial identification. Such hands-on experiences are vital for understanding microbial behavior in environmental samples, as well as their potential implications in both clinical and agricultural settings. #Microbiology #LaboratorySkills #EColiIsolation #EnvironmentalScience #ScientificResearch #PathogenDetection

Access to safe drinking water is vital for our health, homes, industries, and the environment. However, the current methods used by many water testing laboratories, such as most probable number (MPN), or biochemical techniques, can be time-consuming and may not provide rapid and robust identification of potentially pathogenic microorganisms. Fortunately, there is now a solution available to meet the urgent needs of laboratories to quickly identify and confirm microbes in water, including pathogens like 𝘓𝘦𝘨𝘪𝘰𝘯𝘦𝘭𝘭𝘢. This innovative approach does not require second confirmation with classical cultivation, leading to a significant reduction in overall response times by 3-4 days, depending on the organism. Are you ready to streamline your water testing processes? Discover more about MALDI-TOF MS and its ability to expedite turnaround times! https://lnkd.in/dAqUuyWK #BrukerMID #microbiology #WorldWaterDay #WaterSafety #MALDIBiotyper #GOTsirius

🚀 𝐈𝐧𝐭𝐫𝐨𝐝𝐮𝐜𝐢𝐧𝐠 𝐏𝐞𝐧𝐭𝐚𝐓𝐚𝐠 𝐂𝐨𝐧𝐭𝐫𝐨𝐥 𝐏𝐫𝐨𝐭𝐞𝐢𝐧 Effective controls are essential for achieving reliable results, streamlined workflows, and accurate experimental interpretation. When it comes to epitope-tagged proteins, success starts with the right tools in your hands 🛠. That’s why we’re excited to launch 𝐏𝐞𝐧𝐭𝐚𝐓𝐚𝐠 𝐂𝐨𝐧𝐭𝐫𝐨𝐥 𝐏𝐫𝐨𝐭𝐞𝐢𝐧 (Cat. No. P0102) - a versatile positive control protein featuring five widely used epitope tags: FLAG, HA, MBP, Myc, and ALFA. Want to see it in action? Check out the 𝘕𝘢𝘵𝘶𝘳𝘦 𝘊𝘰𝘮𝘮𝘶𝘯𝘪𝘤𝘢𝘵𝘪𝘰𝘯𝘴 publication on the ALFA-System here: https://bit.ly/3BbvcrD. Whether you’re utilizing our #ALFA-tag technology or working with other popular epitope tags, PentaTag Control Protein is the perfect addition to your toolbox. It empowers you to validate Western blotting (WB), dot blot, and immunoprecipitation (IP) outcomes with ease and precision 🎯. View PentaTag Control Protein here 👉 https://bit.ly/415MWzb #ALFAtag #EpitopeTags #WesternBlot #Immunoprecipitation #ResearchTools

Missed our webinar on harvesting and formulation using the µPulse TFF System? Watch the webinar recording: https://hubs.ly/Q02JS8M10 In this webinar, we demonstrate how the µPulse provides a fast, gentle, and automated approach for sample concentration and buffer exchange. We also explore case studies that highlight the efficiency of the µPulse compared to conventional methods for harvesting extracellular VLPs, as well as for the refolding, solubilization, and formulation of various proteins. #LifeSciencesWebinar #Proteins #VLPs #TFF #µPulse #Formulatrix

Xylose-Lysine Deoxycholate Agar (XLD Agar) pic: Salmonella XLD Agar has been recommended for the identification of Enterobacteriaceae. The media formulation does not allow the overgrowth of other organisms over Salmonella and Shigella.Lysine is included to differentiate the Salmonella group from the non-pathogens. Salmonellae rapidly ferment xylose and exhaust the supply. Subsequently lysine is decarboxylate by the enzyme lysine decarboxylase to form amines with reversion to an alkaline pH that mimics the Shigella reaction.Bacteria that decarboxylate lysine to ‘cadaverine’ can be recognized by the appearance of a red colouration around the colonies due to an increase in pH. To add to the differentiating ability of the formulation, an H2S indicator system, consisting of sodium thiosulphate and ferric ammonium citrate, is included for the visualization of hydrogen sulphide produced, resulting in the formation of colonies with black centers.

🔬 Introducing SS Agar for Selective Isolation of Pathogens 🧪 SS Agar is a specialized culture medium designed for the selective isolation and differentiation of Salmonella and Shigella species. Ideal for use in microbiological laboratories, this agar medium provides an effective solution for detecting these pathogens with high accuracy. 🌟 Key Features: Selective Growth: Contains bile salts and other inhibitors to suppress the growth of Gram-positive bacteria and most Enterobacteriaceae, allowing for the selective isolation of Salmonella and Shigella. pH Indicator: Neutral red helps differentiate lactose fermenters (red colonies) from non-fermenters (colorless colonies). Hydrogen Sulfide Detection: Sodium thiosulfate and iron citrate aid in detecting hydrogen sulfide production, resulting in black-centered colonies for certain strains of Salmonella. 🧫 Composition (per liter): Beef Extract: 5.0 g Peptone: 5.0 g Lactose: 10.0 g Sodium Bile Salt No. 3: 8.5 g Sodium Citrate: 8.5 g Sodium Thiosulfate: 8.5 g Iron Citrate: 1.0 g Neutral Red: 0.025 g Brilliant Green: 0.00033 g Agar: 17.0 g pH: 6.9-7.1 🔬 Usage Instructions: Dissolve 63.53 g of SS Agar in 1000 ml of distilled water. Heat to boiling, but no need for autoclaving. Cool to 45-50°C and pour into petri dishes. ✅ Quality Control: Salmonella: Colorless colonies with or without a black center after 18-24 hours at 36±1°C. Shigella: Colorless colonies. Elevate your pathogen detection capabilities with SS Agar and ensure precise results in your microbiological tests! 🌟🔍 #Microbiology #PathogenDetection #LabTech #CultureMedia #Salmonella #Shigella