Aminoverse’s Post

Learn how to enhance protein yield using TeselaGen tools: https://buff.ly/3SI3CrR #synbio #biotech #molecularbiology #AI #bio

🌟 Excited to share the Editor’s Choice Paper, Volume 10Issue 1: A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures. 🥼 This paper is authored by Andreas Ulmer, Stefan Veit, Florian Erdemann, Andreas Freund, Maren Loesch, Attila Teleki, Ahmad A. Zeidan and Ralf Takors. 💡 To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. Welcome to view this paper here👉 : https://lnkd.in/dJYwvHnt. #microbial #metabolomics #lactic_acid_bacteria #Streptococcus_thermophilus #Lactobacillus_bulgaricus #bioprocess_engineering

A very old-fashioned and pedestrian biochemical characterization of an enzyme after heterologous expression: https://lnkd.in/gVzCuGe4. But that kind of work is actually necessary to make sense of the wealth of sequence data that current technologies make available.

🔬 What is LactaZyme®? LactaZyme® is our advanced biosensor enzyme family designed to elevate enzymatic performance and empower developers in precise lactate measurements. Here’s why LactaZyme® stands out: Resilient and Robust: LactaZyme® excels under challenging conditions, becoming your go-to partner in enzymatic innovation. Long Shelf Life: Enjoy consistent performance over time—LactaZyme® remains reliable when you need it most. High Thermal Stability: Whether in extreme heat or cold, LactaZyme® performs reliably, making it your enzymatic workhorse. Oxygen-Independent LDH: Versatility is key—LactaZyme® doesn’t depend on oxygen, broadening its application range. No External Cofactors Needed: Simplify your workflow—LactaZyme® operates independently, without additional cofactors or mediators. Unmatched Precision and Accuracy: Trust LactaZyme® for highly specific and accurate results, with reproducibility boasting an RSD < 10%. Batch-to-Batch Consistency: Every batch is manufactured and certified for homogeneity, meeting ISO standards. 🔗 Learn more about LactaZyme® and how it can transform your work. https://lnkd.in/d6EDq4Kg 📢 Share this post with your network and tag colleagues who should know about LactaZyme®. 🌍 #LactaZyme #Innovation #Science #DirectSens #EnzymeInnovation #Biosensors #DeveloperTools

📢 NEW PAPER ALERT! 📢 Jasmine De Baets wrote a very interesting review about 'Delaying production with prokaryotic inducible expression systems', published in Microbial Cell Factories. If you are interested to learn how two-stage fermentation processes can lead to the efficient production of a molecule of interest then we highly advise you to read the article 👇 Congrats to Jasmine De Baets, Brecht De Paepe & Marjan De Mey! https://lnkd.in/gREp_EeG #synbio #fermentation #quorumsensing #decoupling

Enzyme Immobilization Creative BioMart provides a highly efficient way to go from cell extract or cell culture supernatant to a versatile and robust immobilized pure enzyme preparation. https://lnkd.in/g-vn4MT

Unraveling the Science Behind the "Asparagus Effect" with Advanced GCxGC-TOFMS Technology Have you ever noticed a distinct odor in your urine shortly after enjoying asparagus? This phenomenon, known as the "asparagus effect," is caused by the metabolism of asparagusic acid, a compound unique to asparagus. When metabolized, it transforms into sulfur-containing compounds like methanethiol, dimethyl sulfide, and others that are responsible for this unmistakable aroma. In a recent study, the detection and analysis of these sulfur-containing metabolites were enhanced using GERSTEL's Twister Stir Bar Sorptive Extraction (SBSE) in combination with LECO's GCxGC-TOFMS technology. Here's how this approach revolutionized the process: Reduced Complexity: Traditional extraction techniques required up to 48 hours and the use of hazardous solvents. With SBSE, sample preparation became faster, safer, and solvent-free! Automated Workflow: The integration of the GERSTEL MPS 2–TDU–CIS4 system minimized manual labor through automation, streamlining the entire analysis process. Superior Analysis: LECO's Pegasus GCxGC-TOFMS provided exceptional resolution of analyte peaks, reduced coelutions, and enabled True Signal Deconvolution for accurate compound identification. The result? A faster, more efficient, and environmentally friendly method to study complex metabolites in biological samples. This case underscores the power of advanced analytical technologies in solving intriguing biochemical questions. Have you worked on similar studies or explored the applications of GCxGC-TOFMS in your research? I'd love to hear about your experiences in the comments below! #AsparagusEffect #Metabolomics #GCxGCTOFMS LECO IR Technology Services Pvt Ltd #AnalyticalChemistry #Innovation

Ever feel stressed? Well, so do your bacterial cultures! Give them a break and allow them to grow freely without the burden of production of your molecule of interest by delaying this production. Find out all about the different strategies to obtain this so-called two-stage fermentation in our newest review, published in Microbial Cell Factories! 🦠🏭👩🔬 👇 https://lnkd.in/emtNhUnB

🌟 Excited to announce that our manuscript on the #Micro4All platform and the #MoleculesGateway is now live in the Journal of Natural Products! 🌱🔍 Natural products are essential to drug discovery but challenging to be identified in complex mixtures. Our automated pipeline harmonizes and ranks LC-HRMS data, processing 50 million MS spectra from 7,400 extracts and identifying 150,000 molecules. The #MoleculesGateway enables the recognition of underexplored microbial products, novel analogs, and previously unknown metabolites—revealing untapped chemical diversity. Check it out! 📄🔬 https://lnkd.in/dF8YQ_rJ #NaturalProducts #DrugDiscovery #Metabolomics #ChemicalDiversity #Micro4all

⚙️ Secret to better fermentation-derived products? Strain engineering and optimization! Here are 3 Key Strategies ⤵️ Since synthetic biology’s leap from lab to market, microbial fermentation technology has surged to a $34B industry, projected to hit $60B by 2033. To stay ahead, mastering strain biology and optimization is crucial. Here are 3 powerful strategies to boost titer, yield, and productivity: 🧬 Guided Engineering Approaches: Enhance strain performance using techniques like transcription factor (TF) engineering, membrane/transporter protein optimization, and stress protein engineering. These methods improve protein production, tolerance, and overall productivity. 💻 OptDesign Strategy: Utilize computational modelling to optimize metabolic pathways. OptDesign helps identify minimal genetic changes for efficient production by analyzing differences between wild-type and mutated strains, as seen in high-yield succinate production. 🔎 DySScO Strategy: This approach integrates metabolic and bioprocess engineering to balance yield and growth. It involves three phases—scanning, design, and selection—to fine-tune strains for optimal titer, yield, and productivity. Learn more about these strategies and their impact in my latest mini-deep dive post! (Link in Comments) #StrainEngineering #StrainOptimization #MicrobialFermentation #PrecisionFermentation #SyntheticBiology